Vigro Complete Embryo Flush

Complete Flush Solution is a modification of Holding Plus medium. It is not a substitute for Holding Plus because many of the labile components have been removed to make the solution stable between 15°C and 30°C. However, it is similar because the same buffering system, energy sources, and membrane stabilizers have been retained. Complete Flush Solution and Holding Plus both use HEPES buffering at a concentration that is suited for an air atmosphere and not a CO2 atmosphere. The surfactant properties of proteins are provided by 1mg/mL poly vinyl alcohol (PV-OH). Because proteins also act as heavy metal chelators, Vetoquinol has added other components necessary to fulfill this function. The antibiotics used are gentamycin and kanamycin.

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